While biopsies are a necessary diagnostic tool for EoE, any procedure involving the esophagus could potentially cause irritation. During an upper endoscopy with biopsy, tissue samples are taken from the esophagus to examine eosinophil levels and confirm diagnosis. Since EoE involves inflammation in the esophagus, it's possible that the biopsy procedure could temporarily increase local inflammation and trigger symptoms. If you're experiencing increased symptoms after a procedure, it's important to discuss this with your gastroenterologist to determine if additional treatment adjustments are needed.